1. The 20th NIAES Symposium on the ecological risk assessment of genetically modified crops.

Recently, many genetically modified (GM) crops are developed and utilized rapidly with the progress of biotechnology. Public acceptance of the cultivation of GM crops depends on its assessment of not only the safety of foods and feeds, but also various risks to the ecosystem. Moreover, various types of genes, including the gene resistant to environmental stress, will be transferred into major crops in future. So there are many research subjects to be evaluated on the risk assessment of GM crops.
The symposium, "Ecological Risk Assessment of Genetically Modified Crops" organized by NIAES was held in Tsukuba on November 20, 2000. Following an opening address given by NIAES Director General, 7 speakers presented topics in terms of the ecological risk assessment from viewpoints of various research fields. Approximately 200 individuals including other research institutions, universities and private sectors participated in the symposium.
Participants exchanged information and discussed mainly the results of the urgent investigation on the risk of Bacillus thuingiensis (Bt) corn pollen to non-target butterflies conducted by NIAES, especially 1) the method for the bioassay to detect toxicity of Bt corn pollen to butterflies, 2) the scale effect of the corn fields on the pollen dispersal, and 3) the risk assessment of new GM organisms endowed with the various functions to improve environments. The proceedings of the symposium will be published as the NIAES Research Series.
Symposium schedule: Opening address: K. Minami, Director General, NIAES
1) Situation of GM crops utilization and environmental risk assessment. T. Mitamura, NIAES.
2) Risk assessment of the dispersion of Bt corn pollen against lepidopterous insects. K. Matsuo, NIAES.
3) Interaction between the substances produced by pest resistance crops and insects. O. Saito, National Agricultural Research Center for Kinki, Chugoku, Shikoku Region.
4) Gene flow from cultivated GM crops into the ecosystem. S. Yamane, Monsanto Co., Ltd, Japan.
5) Environmental risk assessment of the viral coat protein gene-transferred crops. Y. Tabei, National Institute of Agro-Biological Resources (NIAR)
6) The horizontal transfer of gene cluster involved in toxin synthesis among bacterial species growing on the plant surface. H. Sawada, NIAES.
7) Environmental risk assessment of next-generation GM crops endowed with new functions such as the resistance to stresses. T. Kayano, NIAR.
8) General discussion: chaired by S. Kawabe, NIAES.

2. FACE 2000 Conference: effects of increasing atmospheric carbon dioxide on plants and ecosystems.

FACE is the acronym of "free-air CO2 enrichment", which is an experimental technique to study terrestrial ecosystems under elevated atmospheric CO2 concentration. In FACE, CO2 is released into the open air to raise the CO2 concentration around a plant canopy without any enclosures, such as plastic covers. FACE enables studies of intact ecosystems under elevated CO2 concentration, whereas studies with other methods can only be done on ecosystems detached and placed in the enclosures, such as greenhouses or controlled chambers. Due to this clear advantage of FACE over the enclosure methods, there are increasing number of FACE experiments set up in various ecosystems around the world. An overview of the FACE and related studies are given in Norby et al. (2001)1), and a list of the FACE projects is posted at the URL: http://public.ornl.gov/face/index.shtml (URL was moved May, 2011).
To review technological and scientific advances of the FACE projects around the world, we held the FACE 2000 international conference at the Tsukuba International Congress Center from June 27 to 30, 2000. The conference was planned to coincide with the final year of the Rice FACE project, which was conducted from 1996 through 2000 under the CREST (Core Research for Evolutional Science and Technology) scheme of Japan Science and Technology Corporation (JST). Preceding the conference, the participants visited the FACE site in Shizukuishi, Iwate Prefecture, and observed the on-going FACE experiment of its final season ( Photo 1 ). Support to the conference was given by JST, NIAES and other research organizations.
Major topics of the conference included FACE technologies, responses of various types of vegetation and ecosystems to increasing atmospheric CO2, and integration across the vegetation types as well as process-based modeling of the vegetation responses. The conference had participants from 14 out of 17 FACE projects around the world, and the participants numbered 107 in all, of which 72 were from Japan, 16 from the USA, 6 from Australia, and 1-2 each from 11 countries in Europe, Asia and South America. Among the FACE projects represented in the conference, the FACE in Arizona, USA and Shizukuishi, Japan studied annual crops (wheat and rice, respectively), those in Switzerland and New Zealand conduced FACE on pastures, and the US FACEs except that in Arizona studied trees and natural vegetation. With the annual crops, a FACE experiment can easily cover a whole life cycle of the crop species, whereas, with perennial species such as trees, a few years of FACE covers only a fraction of the life span of the plant species. The FACE results should therefore be interpreted differently among the experiments with different species and ecosystems. There are, however, common features, such as stimulated photosynthesis, in the plant and ecosystems in their responses to elevated CO2 concentration. To address both differences and similarities between ecosystems, the FACE 2000 Conference had sessions by vegetation types and sessions for synthesizing across them. In the former sessions, findings in FACE were reported and discussed within each vegetation type, whereas, in the latter ones, the FACE findings in various vegetation types were synthesized by each aspect of the ecosystem responses, such as photosynthesis and water relations.
The FACE 2000 Conference inspired communications among the FACE projects around the world, and led to the publication of the special issue, "Rising CO2- future ecosystems", of an international journal, New Phytologist (Vol. 150, No. 2), whose cover page shows the FACE site in Shizukuishi. Many papers in the volume were the outcomes of the conference. As another outcome of the conference, a review of the FACE results on agricultural crops will be published in Advances in Agronomy (Kimball et al., 2002)2). The conference was thus very much productive.

1) Norby, Richard J., Kazuhiko Kobayashi, K., Bruce.A. Kimball (2001). Rising CO2- future ecosystems. New Phytologist 150: 215-221.
2) Kimball, Bruce A., Kazuhiko Kobayashi, Marco Bindi (2002). Responses of agricultural crops to free-air CO2 enrichment. Advances in Agronomy (in press).


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