氏名

宮下清貴　（みやした　きよたか）　Kiyotaka MIYASHITA

所属・職名

理事長

取得学位

農学博士（東京大学） 1979年

現在の業務内容

最近の業績（1995年以降）

（原著論文）

Naoki Osumi, Yoshihiro Kakehashi, Shiho Matsumoto, Kazunari Nagaoka, Junichi Sakai, Kiyotaka Miyashita, Makoto Kimura and Susumu Asakawa (2008) Identification of the gene for disaggregatase from Methanosarcina mazei. Archaea, 2:185-191
Akihiro Saito, Takeshi Fujii, Tomonori Shinya, Naoto Shibuya, Akikazu Ando and Kiyotaka Miyashita (2008) The msiK gene, encoding the ATP-hydrolysing component of N,N9-diacetylchitobiose ABC transporters, is essential for induction of chitinase production in Streptomyces coelicolor A3(2). Microbiology (2008), 154, 3358-3365
Akihiro Saito, Tomonori Shinya, Katsushiro Miyamoto, Tomofumi Yokoyama, Hanae Kaku, Eiichi Minami, Naoto Shibuya, Hiroshi Tsujibo, Yoshiho Nagata, Akikazu Ando, Takeshi Fujii, and Kiyotaka Miyashita (2007) The dasABC Gene Cluster, Adjacent to dasR, Encodes a Novel ABC Transporter for the Uptake of N,N'-Diacetylchitobiose in Streptomyces coelicolor A3(2). Appl. Envir. Microbiol. 73: 3000-3008
Yoriko Sakai, Naoto Ogawa, Takeshi Fujii, Kazuo Sugahara, Kiyotaka Miyashita and Akira Hasebe (2007) 2,4-Dichrolophenoxyacetic Acid-degrading Genes from Bacteria Isolated from Soil in Japan: Spread of Burkholderia cepacia RASC-type Degrading Genes Harbored on Large Plasmids. Microbes and Environments, 22:145-156
Seishi Ikeda, Nozomi Ytow, Hiroshi Ezura, Kiwamu Minamisawa, Kiyotaka Miyashita and Tatsuhito Fujimura (2007) Analysis of Molecular Diversity of Bacterial Chitinase Genes in the Maize Rhizosphere Using Culture-Independent Methods. Microbes and Environments. 22(1) 71-77
Tomokazu Kawase, Shinya Yokikawa, Akihiro Saito, Takeshi Fujii, Naoki Nikaidou, Kiyotaka Miyashita, and Takeshi Watanabe (2006) Comparison of Enzymatic and Antifungal Properties between Family 18 and 19 Chitinases from S. coelicolor A3(2). Biol. Biotechnol. Biochem., 70(4) 988-998
Akihiro Saito, Hanae Kaku, Eiichi Minami, Takeshi Fujii, Akikazu Ando, Yoshiho Nagata, Hilgund Schremnpf, and Kiyotaka Miyashita (2006) An Enzyme-Linked Immnosorbent Assay (ELISA) to Determine the Specificity of the Sugar-Binding Protein NgcE, a Comparison of the ABC transporter for N-Acetylglucosamine in Streptomyces olivaceovirides. Biol. Biotechnol. Biochem. 70(1) 237-242
Gang-hua Lang, Naoto Ogawa, Yusuke Tanaka, Takeshi Fujii, Roberta R. Fulthorpe, Masao Fukuda and Kiyotaka Miyashita (2005) Two kinds of chlorocatechol 1,2-dioxygenase from 2,4-dichlorophenoxyacetate-degrading Sphingomonas sp. strain TFD44. Biochemical and Biophysical Research Communications 332:941-948
Takeshi Fujii, Kiyotaka Miyashita, Ryou Ohtomo and Akihoro Saito (2005) A DNA-Binding Protein Involved in the Regulation of Chitinase Produced in Streptomyces lividans. Biosci. Biotech. Biochem. 69(4) 790-799
Akihiro Saito, Zui Fujimoto, Eiichi Minami, Hiroshi Mizuno, Kiyotaka Miyashita, Hilgund Schrempf and Mitsuru Momma (2004) Crustallization and preliminary X-ray analysis of the Streptomyces olivaceoviridis NgcE binding protein of the ABC transporter for N-acetylglucosamine. Acta Cryst. D60:2358-2360
Tomokazu Kawase, Akihiro Saito, Toshiya Sato, Ryo Kanai, Takeshi Fujii, Naoki Nikaidou, Kiyotaka Miyashita, and Takeshi Watanabe (2004) Distribution and Phylogenetic Analysis of Family 19 Chitinases in Actinobacteria. Appl. Environ. Microbiol. 70: 1135-1144.
N. Ogawa, K. Miyashita and A.M. Chakrabarty (2003) Microbial Genes and Enzymes in the Degradation of Chlorinated Compounds. The Chemical Record, 3:158-171
A. Saito, T. Fujii, K. Miyashita (2003) Distribution and evolution of chitinase genes in Streptomyces species: involvement of gene-duplication and domain-deletion. Antonie van Leeuwenhoek 84(1) 7-16
Shin Muraoka, Rumi Okumura, Yoshitaka Uragami, Takamasa Nonaka, Naoto Ogawa, Kiyotaka Miyashita and Toshiya Senda (2003) Purification and crystallization of a LysR-type transcriptional regulator from Ralstonia eutropha NH9. Protein & Peptide Letters. (in press) http://www.bentham.org/ Volume 10, No. 3, 2003 Pp.325-329
Shin Muraoka, Rumi Okumura, Naoto Ogawa, Takamasa Nonaka, Kiyotaka Miyashita and Toshiya Senda (2003) Crystal Structure of a Full-length LysR-type Transcriptional Regulator, CbnR: Unusual Combination of Two Subunit Forms and Molecular Bases for Causing and Changing DNA Bend. J. Mol. Biol., 328: 555-566
K. Suzuki, A. Ichimura, N. Ogawa, A. Hasebe, and K. Miyashita (2002) Differential expression of two catechol 1,2-dioxygenases in Burkholderia sp. TH2. J. Bacteriol. 184: 5714-5722
M. Shimizu, T. Kimura, T. Koyama, K. Suzuki, N. Ogawa, K. Miyashita, K. Sakka, and K. Ohmiya (2002) Molecular Breeding of Transgenic Rice Plants Expressing a Bacterial Chlorocatechol Dioxygenase Gene. Appl. Envir. Microbiol., 68: 4061-4066
S. Liu, N. Ogawa and K. Miyashita (2001) The chlorocatechol degradative genes, tfdT-CDEF, of Burkholderia sp. strain NK8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols, Gene 268: 207-214
H. Kong, M. Shimosaka, Y. Ando, K. Nishiyama, T. Fujii, and K. Miyashita (2001) Species-specific distribution of a modular family 19 chitinase gene in Burkholderia gladioli. FEMS Microbiology Ecology 1270: 1-5
A. Saito, K. Miyashita, G. Biukovic, and H. Schrempf (2001) Characteristics of a Streptomyces coelicolor A3(2) extracellular protein targeting chitin and chitosan. Appl. Environ. Microbiol. 67: 1268-1273
K. Suzuki, N. Ogawa, and K. Miyashita (2001) Expression of a 2-halobenzoate dioxygenase genes (cbdSABC) involved in the degradation of benzoate and 2-halobenzoate in Burkholderia sp. TH2. Gene 262: 137-145
P.B. Francisco, Jr, N. Ogawa, K. Suzuki and K. Miyashita (2001) The chlorobenzoatedioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates. Microbiology 147: 121-133
A. Saito, M. Ishizaka, P. B. Francisco, Jr., T. Fujii, and K. Miyashita (2000) Transcriptional co-regulation of five chitinase genes scattered on the Streptomyces coelicolor A3(2) chromosome. Microbiology 146: 2937-2946
H. Ueno and K. Miyashita (2000) Inductive production of chitinolytic enzymes in soil microcosms using chitin, other carbon-sources, and chitinase-producing Streptomyces. Soil Sci. Plant Nutr. 46: 863-871
M. Yamasaki, K. Miyashita, J. Cullum, and H. Kinashi (2000) A Complex insertionsequence cluster at a point of interaction between the linear plasmid SCP1 and the linear chromosome of Streptomyces coelicolor A3(2). J. Bacteriol. 182: 3104-3110
K. Miyashita, T. Fujii, A. Saito (2000) The induction and repression of a Streptomyces lividnas chitinase gene promoter in response to various carbon sources. Biosci. Biotechnol. Biochem. 64:39-43
N. Ogawa, S. M. McFall, T. J. Klem, K. Miyashita, A.M. Chakrabarty (1999) Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9. J. Bacteriol. 181: 6697-6705
T. Watanabe, R. Kanai, T. Kawase, T. Tanabe, M. Mitsutomi, S. Sakuda, K. Miyashita (1999) Family 19 chitinases of Streptomyces species: characterization and distribution. Microbiology 145:3353-63
A. Saito, T. Fujii, T. Yoneyama, M. Redenbach, T. Ono, T. Watanabe, and K.Miyashita (1999) Highly-multiplicity of chitinase genes in Streptomyces coelicolor A3(2). Biosci. Biotechnol. Biochem. 63: 710-718
N. Ogawa, and K. Miyashita (1999) The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-dagrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on a 3-chlorobenzoate. Appl. Environ. Microbiol. 65:724-731
A. Saito, T. Fujii, T. Yoneyama, and K. Miyashita (1998) glkA is involved in glucose repression of chitinase production in Streptomyces lividans. J. Bacteriol. 180: 2911-2914
K. Miyashita, T. Fujii, A. Watanabe, and H. Ueno (1997) Nucleotide sequence and expression of a gene for a chitinase (chiB) from Streptomyces lividans. J. Ferment. Bioeng. 83: 26-31
S. Tsushima, A. Hasebe, Y. Komoto, J. P. Carter, K. Miyashita, K. Yokoyama, and R.W. Pickup (1995) Detection of genetically engineered microorganisms in paddy soil using a simple and rapid nested polymerase chain reaction method. Soil Biol. Biochem. 27: 219-227
N. Ogawa and K. Miyashita (1995) Recombination of a 3-chlorobenzoate catabolic Plasmid from Alclaligenes eutrophus NH9 mediated by direct repeat elements Appl. Environ. Microbiol. 61: 3788-3795

（総説・解説）

宮下清貴(2009) 今日の環境問題と土壌改良資材．農業及び園芸　84:197-202
N. Ogawa, K. Miyashita, and A.M. Chakrabarty (2003) Microbial Genes and Enzymes in the Degradation of Chlorinated Compounds. The Chemical Record, 3: 158-171
Saito, T. Fujii and K. Miyashita (1999) Chitinase system in Streptomyces. Actinomycetologica, 13:1-10
宮下清貴(1998) 土壌微生物機能の活用．農業および園芸　73: 145-190
宮下清貴(1998) 土壌微生物の機能利用と土壌微生物．季刊肥料　79号: 42-52
宮下清貴、藤井毅、小川直人(1996) 土壌の腐生性細菌から見たゲノム研究と遺伝生態研究の接点　IGEシリーズ23, p.137-149「遺伝生態情報の可能性」東北大学遺伝生態研究センター

（著書）

改訂版　土の微生物学　(2008)　服部勉、宮下清貴、齋藤明広　養賢堂
新・土の微生物 (9) 放線菌の機能と働き (2003) 日本土壌微生物学会編博友社
微生物の資材化研究の最前線 (共編、共著) (2000) 「有害物質のバイオレメディエーション」p.301-313 ソフトサイエンス社
新版微生物学実験法 (共著) (1999) 「DNAプローブ法による検出」 p.277-280 講談社サイエンティフィク、講談社
新・土の微生物 (3) 遺伝子と土壌微生物 (編集、共著) (1998) 「土壌環境と微生物の遺伝子発現調節」 p.121-152 博友社
農業環境を守る微生物 (共著) (1998) 「汚染土壌のバイオリメディエーション」 p. 264-286 家の光協会
未来に広がる微生物利用 (自然の中の人間シリーズ微生物と人間編第7巻) (1997) 農山漁村文化協会
バイオレメディエーションの実際技術 (共著) (1996) 「農林水産分野におけるバイオレメディエーション」 p.18-26 シーエムシー
土の微生物学 (服部勉・宮下清貴共著) (1996) 養賢堂