研究者情報 (国立研究開発法人農業環境技術研究所)
2015年4月現在
氏名
吉田 重信 (よしだ しげのぶ) YOSHIDA, Shigenobu
所属・職名
生物生態機能研究領域 主任研究員
現在の研究内容
2006年4月より、各種植物の病原糸状菌および細菌の分類・同定および機能の解析を行うとともに、植物体上等の農業環境に生息する微生物の効果的な利用のために、生息微生物の生理生態学的な解析を行っている。
最近の業績(2000年以降)
(原著論文)
- Yoshida, S., Koitabashi, M., Nakamura, J., Fukuoka, T., Sakai, H., Abe, M., Kitamoto, D. and H. Kitamoto, H. Effects of biosurfactants, mannosylerythritol lipids, on the hydrophobicity of solid surfaces and infection behaviors of plant pathogenic fungi. J. Appl. Microbiol. in press.
- Fukuoka, T., Yoshida, S., Nakamura, J., Koitabashi, M., Sakai, H., Abe, M., Kitamoto, D. and Kitamoto, H. Agricultural application of yeast glycolipid biosurfactant, mannosylerythritol lipid, to the spreading agents. J. Oleo Sci. in press.
- Ueda, H., Mitsuhara, I., Tabata, J., Kugimiya, S., Watanabe, T., Suzuki, K., Yoshida, S. and Kitamoto, H. Extracellular esterases of phylloplane yeast Pseudozyma antarctica induce defect on cuticle layer structure and water-holding ability of plant leaves. Appl. Micobiol. Biotechnol. in press.
- Matsushita, Y., Yamamura, K., Morimoto, S., Bao, Z., Kurose, D., Sato, I., Yoshida, S. and Tsushima, S. Analysis of variations in band positions for normalization in across-gel denaturing gradient gel electrophoresis. J. Microbiol. Methods. 112:11-20. 2015.
- Matsushita, Y., Bao, Z., Kurose, D., Okada, H., Takemoto, S., Sawada, A., Nagase, H., Takano, M., Murakami, H., Koitabashi, M.., Yoshida, S., Saito, M., Sano, T. and Tsushima, S. Community structure, diversity, and species dominance of bacteria, fungi, and nematodes from naturally and conventionally farmed soil: a case study on Japanese apple orchards. Organic Agriculture. 5: 11-28. 2015.
- 長瀬陽香・丹羽理恵子・松下裕子・池田健太郎・山岸菜穂・串田篤彦・岡田浩明・吉田重信・對馬誠也 (2015) 圃場におけるハクサイ黄化病発生程度とPCR-DGGE法に基づく土壌微生物相の関係. 日本植物病理学会報.81: 9-21.2015.
- Yoshida, S., Morita, M., Shinozaki, Y., Watanabe, T., Sameshima-Yamashita, Y., Koitabashi, M., Kitamonto, D. and Kitamoto, H. Mannosylerythritol lipids secreted by phyllosphere yeast Pseudozyma antarctica is associated with its filamentous growth and propagation on plant surfaces. Appl. Microbiol. Biotechnol. 98: 6419-6429. 2014.
- Sato, I., Yoshida, S., Iwamoto, Y., Aino, M., Hyakumachi, M., Shimizu, M., Takahashi, H., Ando, S. and Tsushima, S. Suppressive potential of Paenibacillus strains isolated from the tomato phyllosphere against Fusarium crown and root rot of tomato. Microbes Environ. 29: 168-177. 2014.
- Watanabe, T., Shinozaki, Y., Suzuki, K., Koitabashi, M., Yoshida, S. Sameshima-Yamashita, Y. and Kitamoto, H.K. Production of a biodegradable plastic-degrading enzyme from cheese whey by the phyllosphere yeast Pseudozyma antarctica GB-4(1)W. J. Biosci. Bioeng. 118: 183-187.
- Suzuki, K., Tsujimoto-Noguchi, M., Shinozaki, Y., Koitabashi, M., Sameshima-Yamashita, Y., Yoshida, S., Fujii, T. and Kitamoto, H.K. Purification, characterization, and cloning of the gene for a biodegradable plastic-degrading enzyme from Paraphoma-related fungal strain B47-9. Appl. Microbiol. Biotechnol. 98: 4457-4465. 2014.
- Takahashi, H., Nakaho, K., Ishihara, T., Ando, S., Wada, T., Kanayama, Y., Asano, S., Yoshida, S., Tsushima, S. and Hyakumachi, M. Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum. Plant Cell Rep. 33: 99-110. 2014.
- Watanabe, T., Shinozaki, Y., Yoshida, S., Koitabashi, M., Sameshima-Yamashita, Y., Fujii, T., Fukuoka, T. and Kitamoto, H.K. Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics. J. Biosci. Bioeng.117: 325-329. 2014.
- 岩本豊・松浦克成・佐藤育男・吉田重信・對馬誠也・相野公孝 Paenibacillus 属細菌のトマト根腐萎凋病に対するほ場における防除効果.関西病虫害研究会報.55: 67-69. 2013.
- 小板橋基夫・月星隆雄・篠原弘亮・吉田重信・對馬誠也 農業環境技術研究所が所蔵する微生物さく葉標本目録のWeb公開.日本植物分類学会誌 分類.13: 137-141. 2013.
- Shinozaki, Y., Kikkawa, Y., Sato, S., Fukuoka, T., Watanabe, T., Yoshida, S., Nakajima-Kambe, T. and Kitamoto, HK. Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study. Appl. Microbiol. Biotechnol. 97: 8591-8598. 2013.
- Hyakumachi, M., Nishimura, M., Arakawa, T., Asano, T., Yoshida, S., Tsushima, S. and Takahashi, H. Bacillus thuringiensis suppresses bacterial wilt disease caused by Ralstonia solanacearum with systemic induction of defense-related gene expression in tomato. Microbes Environ. 28: 128-134. 2013.
- Shinozaki, Y., Morita, T., Cao, X-H., Yoshida, S., Koitrabashi, M., Watanabe, T., Suzuki, K., Yamashida-Sameshima, Y., Fujii, T. and Kitamoto, HK. Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. Appl. Microbiol. Biotechnol. 97: 2951-2959. 2013.
- Ito, M, Sato, I., Ishizaka, M., Yoshida, S., Koitabashi, M., Yoshida, S. and Tsushima, S. A bacterial cytochrome P450 system catabolizing the Fusarium toxin deoxynivalenol. Appl. Environ. Microbiol. 79: 1619-1628. 2012.
- Koitabashi, M., Noguchi-Tsujimoto, M., Sameshima-Yamashita, Y., Hiradate, S., Suzuki, K., Yoshida, S., Watanabe, T., Shinozaki, Y., Tsushima, S. and Kitamoto, H. Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants. AMB Express 2: 40, doi: 10.1186/2191-0855-2-40. 2012.
- Yoshida, S., Oba, A., Liang, Y-M., Koitabashi, M. and Tsushima, S. Specificity of Pseudomonas isolates on healthy and Fusarium head-blight-infected spikelets of wheat heads. Microbial Ecol. 64:214-225. 2012
- Ito, M., Sato, I., Koitabashi, M., Yoshida, S.. Imai, M. and Tsushima, S. A novel actinomycete derived from wheat heads degrades deoxynivalenol in the grain of wheat and barley affected by Fusarium head blight. Appl. Microbiol. Biotechnol. 96: 1059-1070. 2012
- Sato, I., Ito, M. Ishizaka, M., Ikunaga, Y., Sato, Y., Yoshida, S., Koitabashi, M. and Tsushima, S. Thirteen novel deoxynivalenol‐degrading bacteria are classified into just two genera with distinct degradation mechanisms. FEMS Microbiol. Lett. 327: 110-117. 2012
- Kitamoto, H., Shinozaki, Y., Cao, X-H., Morita, T., Konishi, M., Tago, K., Kajiwara, H., Koitabashi, M., Yoshida, S., Watanabe, T., Sameshima-Yamashita, Y., Nakajima-Kambe, T. and Tsushima, S. Phyllosphere yeasts rapidly break down biodegradable plastics. AMB Express 1:44, doi:10.1186/2191-0855-1-44. 2011.
- Shinohara H., Yoshida, S., Enya, J., Watanabe, Y., Tsukiboshi, T., Negishi, H. and Tsushima, S. Culturable bacterial communities on leaf sheaths and panicles of rice plants in Japan. Folia Microbiology 56: 505-517. 2011.
- Koitabashi, M., Yoshida, S. and Tsushima, S. Labor-saving preservation of powdery mildew of strawberry by sterilized seedling culture, JARQ 45: 405-409. 2011.
- Niwa, R., Yoshida, S., and Tsushima, S. Method for simple and rapid enumeration of total epiphytic bacteria in the washing solution of rice plants. Can. J. Microbiol. 57: 62-67. 2011.
- Ikunaga, Y., Sato, I., Grond, S., Numaziri, N., Yoshida, S., Yamaya H., Hiradate, S., Hasegawa, M., Toshima, H., Koitabashi, M., Ito, M. Karlovsky, P. and Tsushima, S. Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. Appl. Microbiol. Biotechnol. 89: 419-427. 2010.
- Yoshida, S., Ogawa, N., Fujii, H. and Tsushima, S. Enhanced biofilm formation and 3-chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO1. J. Appl. Microbiol. 106: 790-800. 2009.
- Umemoto, S., Odake, Y., Takeuchi, T., Yoshida, S., Tsushima, S. and Koitabashi, M. Blue mold of tomato caused by Penicillium oxalicum in Japan. J. Gen. Plant Pathol. 75: 399-400. 2009.
- 大場淳司・吉田重信・對馬誠也・生井恒雄 赤かび病抵抗性の異なるコムギ2品種における赤かび病発生とデオキシニバレノール蓄積に及ぼす感染時期の影響.日植病報.75: 93-101. 2009.
- Enya, J., Togawa, T., Takeuchi, S., Yoshida, S., Tsushima, S., Arie, T. and Sakai, T. Biological and phylogenetic characterization of Fusarium oxysporum complex, which causes yellows on Brassica spp., and proposal of Fusarium oxysporum f. sp. rapae, a novel forma specialis pathogenic on Brassica rapa L. in Japan. Phytopathology 98: 475-483. 2008.
- Yoshida, S., Tsukiboshi, T., Shinohara, H., Koitabashi, M. and Tsushima, S. Occurrence and development of Colletotrichum acutatum on symptomless blueberry bushes. Plant Pathology 56: 871-877. 2007.
- Enya, J., Koitabashi, M., Shinohara, H., Yoshida, S., Tsukiboshi, T., Negishi, H., Suyama, K. and Tsushima, S. Phylogenetic diversities of dominant culturable Bacillus, Pseudomonas and Pantoea species on tomato leaves and their possibility as biological control agents. J. Phytopathology 155: 446-453. 2007.
- Yoshida, S., Tsukiboshi, T., Arie, T., Shinohara, H., Koitabashi, M. and Tsushima, S. Inhabitancy and colonization on healthy rice plants by Glomerella cingulata. J. Phytopathology 155: 38-44. 2007.
- Enya, J., Shinohara, H., Yoshida, S., Tsukiboshi, T., Negishi, H., Suyama, K. and Tsushima, S. Culturable leaf-associated bacteria on tomato plants and their potential as biological control agents. Microbial Ecology 53: 524-536. 2007.
- 吉田重信 農業環境中に生息する微生物のバイオフィルムとその役割. 植物細菌病談話会論文集 24: 85-99. 2007.
- Yoshida, S., Kinkel, L. L., Shinohara, H., Numajiri, N., Hiradate, S., Koitabashi, M., Suyama, K., Negishi, H. and Tsushima, S. Production of quorum sensing-related signal molecules by epiphytic bacteria inhabiting wheat heads. Can. J. Microbiol. 52: 411-418. 2006.
- Tsukiboshi, T., Chung, W. H. and Yoshida, S.. Cochliobolus heveicola sp.nov. (Bipolaris heveae) causes brown stripe of bermudagrass and Zoysia grass. Mycoscience 45: 17-21. 2005.
- Yoshida, S., Suzuki, F., Tsukiboshi, T. and Shinohara, H. Cloning and characterization of a gene rpg1 encoding polygalacturonase of Rhizopus oryzae. Mycological Research 108: 1407-1414.2004.
- 月星隆雄・吉田重信・篠原弘亮・對馬誠也 日本野生植物寄生・共生菌類目録の作成とWeb公開. 日本植物分類学会誌(分類)4: 77-80. 2004.
- Yoshida, S., Tsuyumu, S. and Tsukiboshi, T. Macerating enzymes produced by Rhizopus oryzae in infected mulberry roots.J. Phytopathology 151: 436-441. 2003.
- Yoshida, S. and Tsukiboshi, T. Shoot blight and leaf spot of blueberry anthracnose caused by Colletotrichum acutatum.J. Gen. Plant Pathol. 68: 246-248. 2002.
- Hiradate, S., Yoshida, S., Sugie, H., Yada, H. and Fujii, H.Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2. Phytochemistry 61: 693-698. 2002.
- Yoshida, S., Hiradate, S., Tsukamoto, T., Hatakeda, K. and Shirata, A.Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. Phytopathology 91: 181-187. 2001.
- Yoshida, S., Murakami, R., Watanabe, T. and Koyama, A. Rhizopus rot disease of mulberry grafted saplings caused by Rhizopus oryzae Went & Prinsen Geerligs. J. Gen. Plant Pathol. 67: 291-293 . 2001.
- Yoshida, S. and Shirata, A. Biotin induces sporulation of mulberry anthracnose fungus, Colletotrichum dematium. J. Gen. Plant Pathol. 66: 117-122. 2000.
- Yoshida, S., Hiradate, S., Fujii, H. and Shirata, A. Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves. Phytopathology 90: 285-291. 2000.
- Yoshida, S.Yoshida, S., R. Murakami, T. Ichihashi and M. Yazawa. Changes in the susceptibility of mulberry leaves applied differing amounts of fertilizer to phytopathogenic fungi. J. Seric. Sci. Jpn. 69: 305-311. 2000.
(総説)
- 吉田重信 (2014) 作物の虫害と病害の両方に有効な微生物農薬開発の可能性. バイオインダストリー 31: 29-33. 2014.
- 池田健太郎・酒井宏・田中一史・柴田聡・坂野真平・藤村真・渡邊健・山岸菜穂・藤永真史・小木曽秀紀・串田篤彦・吉田重信・對馬誠也 ハクサイ黄化病菌 Verticillium dahliae と V. longisporum の分布と圃場診断に基づいた防除. 植物防疫、68: 30-33. 2014.
- 小板橋基夫・月星隆雄・篠原弘亮・吉田重信・對馬誠也 農業環境技術研究所が所蔵する微生物さく葉標本目録のWeb公開.日本植物分類学会誌 分類.13: 137-141. 2013.
- 吉田重信・對馬誠也 植物病害に対する微生物農薬の研究開発における課題と展望.化学と生物 51: 541-547. 2013.
- 北本宏子・小板橋基夫・篠崎由紀子・渡部貴志・山下結香・吉田重信 植物常在菌の酵素で生分解性プラスチックを素早く分解する.BioPla Journal 49:16-21. 2013.
- 北本宏子・篠崎由紀子・鈴木健・小板橋基夫・吉田重信・渡部貴志・山下結香 生分解性プラスチックを酵素で急速分解する技術.工業材料 61: 35-38. 2013.
- 吉田重信 異種微生物間の相互作用およびその生物防除への活用の可能性.植物防疫 63: 619-623. 2009.
- 吉田重信 クワ炭疽病の生理・生態学的研究.農業生物資源研究所研究資料 1: 1-78. 2002.
- Yoshida, S., Shirata, A. and Hiradate, S. Ecological characteristics and biological control of mulberry anthracnose. JARQ 36: 89-95. 2002.
- 吉田重信 クワ炭疽病の発生生態と拮抗細菌による発病抑制.植物防疫 56: 349-352. 2002.
(著書)
- 吉田重信 Bacillus属細菌、「土壌微生物実験法第3版」(養賢堂)pp. 228-230. 2013.
- 吉田重信 異種微生物間の相互作用(細菌と菌類および細菌と細菌)、「微生物と植物の相互作用-病害と生物防除-」(ソフトサイエンス社)pp. 341-346. 2009.
- 吉田重信 キーワード:「クオラムセンシング」、「微生物と植物の相互作用-病害と生物防除-」(ソフトサイエンス社)p.68. 2009.
- 吉田重信 キーワード:「バイオフィルム」、「微生物と植物の相互作用-病害と生物防除-」(ソフトサイエンス社)p.68. 2009.
- 吉田重信 Bacillus属細菌が分泌する抗菌成分による炭疽病菌の生育抑制作用、「農業生態系の保全に向けた生物機能の活用」(養賢堂)pp. 135-149. 2005.
- 吉田重信 quorum sensingとquorum quenching-植物病原細菌のユニークな発病戦略とその防除のための利用-、「作物保護の新展開-バイオサイエンスのかけはし-」(ソフトサイエンス社)pp. 90-92. 2004
(特許)
- 北本宏子・小板橋基夫・吉田重信・渡部貴志・森田友岳(PCT/JP2014/ 050233.) 高効率な異種タンパク質の製造方法..2014.
- 北本宏子・渡部貴志・森田友岳・小板橋基夫・吉田重信 (特願2013-2018) 高効率な異種タンパク質の製造方法.2013.
- 北本宏子・釘宮聡一・植田浩一・黒瀬大介・吉田重信・田端純・小板橋基夫・渡部貴志・篠崎由紀子 (特願2012-288082) 除草用酵素含有組成物及び有害植物の駆除方法.2012.
- 吉田重信・小板橋基夫・對馬誠也・平舘俊太郎 (特願2011-276731)植物生息微生物由来の紫外線吸収剤組成物.2011.
- 北本宏子・小板橋基夫・吉田重信・渡部貴志(特願2011-186786)葉面酵母の生分解性プラスチック分解酵母の大量生産方法.2011.
- 吉田重信・北本宏子(特願2011-119946)微生物農薬製剤.2011.
- 吉田重信・鈴木文彦・月星隆雄・篠原弘亮・對馬誠也(特許第4168130号) リゾプス属糸状菌のポリガラクツロナーゼおよびポリガラクツロナーゼ遺伝子.2008.
- 對馬誠也・小板橋基夫・吉田重信・生長陽子 (特開2008-220184)デオキシニバレノールを分解する新規微生物.2008.
- 對馬誠也・小板橋基夫・吉田重信・生長陽子 (特開2008-220179)デオキシニバレノールを分解する新規微生物.2008.
- 吉田重信 特許第3482462号 微生物を定着させたおからからなる植物保護剤及びそれを用いた植物病害の防除方法. 2003.