Name

TAKEMOTO, Shuhei

Position

Environmental Biofunction Division
NIAES Postdoctoral Research Fellow

Research activities

Since 2008, I have engaged in the development of a practical analysis method for soil nematode communities on the basis of genomic information. See details at the official site of the project “Development of soil biodiversity analysis system with environmental DNA” (eDNA Project ; (http://www.niaes.affrc.go.jp/project/edna/edna_jp/index.html).

Education

March, 2007	Ph. D. in Agriculture, Kyoto University, Kyoto, Japan Studies on the virulence evolution of the pinewood nematode, Bursaphelenchus xylophilus.
March, 2004	Master in Agriculture, Kyoto University, Kyoto, Japan
March, 2002	Bachelor in Agriculture, Kyoto University, Kyoto, Japan

Professional experience & research topics

April, 2008 - Present	NIAES Postdoctoral Research Fellow, National Institute for Agro-Environmental Sciences, Tsukuba, Japan Development of the standard analytical system for soil-nematode communities based on PCR-DGGE, and its application to paddy-field sediment.
April, 2007 - March, 2008	Research assistant, Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Tsukuba, Japan Taxonomic studies on the fungal genus Rosellinia including the white root rot fungus Rosellinia necatrix. Etiological studies of a white-rot basidiomycete, Schizophyllum commune.
	External Researcher, Kanagawa Prefectural Museum of Natural History, Odawara, Japan Flora of Rosellinia and related fungi in Kanagawa Prefecture.

Society memberships

Ecological Society of Japan
The Mycological Society of Japan
The Japanese Forest Society
The Japanese Nematological Society
The Japanese Society of Applied Entomology and Zoology
The Japanese Society of Soil Microbiology
Tree Health Research Society, Japan

Publication (2005-present):

(Original Papers)

Shuhei Takemoto and Hiroaki Okada (2009) Suppressed-priming PCR, a novel concept of DNA quantification based on PCR kinetics. Bioscience, Biotechnology, and Biochemistry. (accepted)
Shuhei Takemoto, Yosuke Degawa and Hitoshi Nakamura (2009) An undescribed Rosellinia necatrix-like fungus found in Kanagawa Prefecture. Tree and Forest Health. 13(2): 79-80 (In Japanese with English title).
Shuhei Takemoto, Hitoshi Nakamura and Yosuke Degawa (2009) The first record of Rosellinia aquila in Kanagawa Prefecture and the analysis of morphological variation among the collections. Bulletin of the Kanagawa Prefectural Museum (Natural Science). 38: 21-29.
Shuhei Takemoto, Hitoshi Nakamura, Atsuko Sasaki and Takanori Shimane (2009) Rosellinia compacta, a new species similar to the white root rot fungus Rosellinia necatrix. Mycologia. 101(1): 84-94.
Erwin, Shuhei Takemoto, Won-Joung Hwang, Miyuki Takeuchi, Takao Itoh and Yuji Imamura (2008) Anatomical characterization of decayed wood in standing light red meranti and identification of the fungi isolated from the decayed area. Journal of Wood Science. 54(3): 233-241.
Ai Nozaki, Masahide Kobayashi, Haruo Kinuura, Syuhei Takemoto and Kazuyoshi Futai (2007) Inoculation of several fungi on Quercus crispula killed by the infection of Platypus quercivorus. Applied Forest Science. 16: 1-9 (In Japanese with English summary).
Rina Sriwati, Shuhei Takemoto and Kazuyoshi Futai (2007) Cohabitation of the pine wood nematode and fungal species in pine trees inoculated with Bursaphelenchus xylophilus. Nematology. 9(1): 77-86.
Shuhei Takemoto and Kazuyoshi Futai (2007) Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A locus and the field detection of polymorphic populations. Applied Entomology and Zoology. 42(2): 247-253.
Rina Sriwati, Shuhei Takemoto and Kazuyoshi Futai (2006) Seasonal changes of nematode fauna in pine trees killed by the pinewood nematode, Bursaphelenchus xylophilus. Japanese Journal of Nematology. 36(2): 87-100.
Manuel Melo Mota, Shuhei Takemoto, Yuko Takeuchi, Naoki Hara1 and Kazuyoshi Futai (2006) Comparative studies between Portuguese and Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus. Journal of Nematology. 38(4): 429-433.
Long Ke Phan, Shuhei Takemoto and Kazuyoshi Futai (2006) Steinernema ashiuense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Japan. Nematology. 8(5): 681-690.
Shuhei Takemoto, Natsumi Kanzaki and Kazuyoshi Futai (2005) PCR-RFLP image analysis - a practical method for estimating isolate-specific allele frequency in a population consisting of two different strains of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelencoididae). Applied Entomology and Zoology. 40(3): 529-535.

(Books)

Shuhei Takemoto and Kazuyoshi Futai (2008) Rapidity of disease development seems to result in high mortality - insight from an inoculation test using hybridized populations between a virulent and avirulent isolates of Bursaphelenchus xylophilus. In Pine wilt disease: A worldwide threat to forest ecosystems (Mota MM and Vieira P eds), Springer-Verlag Dordrecht, pp303-311.
Shuhei Takemoto (2008) Population ecology of Bursaphelenchus xylophilus. In Pine wilt disease (Zhao BG et al eds), Springer-Verlag Tokyo, pp105-122.

(Reviews and Commentaries)

Shuhei Takemoto and Noritoshi Maehara (2007) Recent studies on pine wilt disease - a report of the 118th annual meeting of The Japanese Forest Society -. Forest Pest. 56(4): 137-140 (in Japanese).